Field Name,Data Type,Description,Value Set,Units,Submission Requirement,Dependent Fields ,,,,,, Reporter,,,,,, reporting_jurisdiction,category,"The CDC Epidemiology and Laboratory Capacity (ELC) jurisdiction, most frequently a state, reporting these data (2-letter abbreviation)",[See Value Sets: vs_reporting_jurisdiction],[none],Required,None ,,,,,, Collection Site,,,,,, site_id,<#####-###-##-##-##>,Nationally unique sampling site identifier assigned following NWSS format. <5 digit county FIPS code for WWTP address>-<3 digit facility code>-<2 digit interceptor and sewershed code>-<2 digit subsewershed code>-<2 digit site code>,,,Required,None county_names,list (comma-separated integers),"5-digit numeric FIPS codes of all counties and county equivalents served by this sampling site (i.e., served by this wastewater treatment plant or, if 'sample_location' is ""upstream"", then by this upstream location). Note that most jurisdictions are covered by counties, but some are covered by county equivalents, such as independent cities, parishes, or census areas.",[5-digit integers],[none],Required,None zipcode,ZIP code (#####),Zip code in which this sampling site is physically located (one 5-digit zip code),[5-digit integers],[none],Required,None population_served,integer,"Estimated number of persons served by this sampling site (i.e., served by this wastewater treatment plant or, if 'sample_location' is ""upstream"", then by this upstream location)",[greater than or equal to 0],[none],Required,None sewage_travel_time,float,"What is the approximate sewage travel time, on average, from sewage source to this sampling site (i.e., this wastewater treatment plant or, if 'sample_location' is ""upstream"", then this upstream location)? This should be specified as a duration in hours, not a time of day.","[greater than or equal to 0]; [empty]",Hours,Not required,None sample_location,category,"Sample collection location in the wastewater system, whether at a wastewater treatment plant (or other community level treatment infrastructure such as community-scale septic) or upstream in the wastewater system",[See Value Sets: vs_sample_location],[none],Required,"If 'sample_location' is ""upstream"", specify in 'sample_location_specify'" sample_location_specify,string,"If 'sample_location' is ""upstream"", specify the collection location in the wastewater system; an arbitrary name may be used if you do not wish to disclose the real name. ","[string, length less than or equal to 40 characters]; [empty]",[none],Required,"If sample_location is ""upstream"", then this must have a non-empty value" institution_type,category,"If this sample represents wastewater from a single institution, facility, or building, specify the institution type; otherwise, specify ""not institution specific""",[See Value Sets: vs_institution_type],[none],Required,None ,,,,,, WWTP,,,,,, epaid,NPDES permit number (<2-letter abbreviation><#######>),"NPDES permit number for the wastewater treatment plant specified in 'wwtp_name'. If 'upstream location' use the NPDES permit number for the wastewater treatment plant in which the water flows. If facility does not have an NPDES permit number, enter -1. ",[NPDES permit number]; -1 if not permitted,[none],Required,None epa_registry_id,EPA Registry ID (<############>),"EPA registry ID for the wastewater treatment plant specified in 'wwtp_name'. If 'upstream location' use the registry ID number for the wastewater treatment plant in which the water flows. If the water does not flow to a treatment plant, enter -1. ",[EPA Registry ID]; -1 if not registered with EPA,[none],Not required,None wwtp_name,string,"The name of the Wastewater Treatment Plant (WWTP) to which this wastewater flows. If this wastewater does not flow to a WWTP, specify an identifiable name for the septic or other treatment system to which this wastewater flows. An arbitrary name may be used if you do not wish to disclose the real name.","[string, length less than or equal to 40 characters]",[none],Required,None wwtp_jurisdiction,category,"State, DC, US territory, or Freely Associated State jurisdiction name (2-letter abbreviation) in which the wastewater treatment plant provided in 'wwtp_name' is located",[See Value Sets: vs_wwtp_jurisdiction],[none],Required,None capacity_mgd,float,"Wastewater treatment plant design capacity. This should be the capacity for which the plant is permitted. If 'upstream location', use the design capacity for the wastewater treatment plant to which the water flows.",[greater than or equal to 0],Million gallons per day (MGD),Required,None industrial_input,float,Approximate average percentage of wastewater from industrial sources that is received by the wastewater treatment plant specified in 'wwtp_name',"[0-100]; [empty]",percent,Not required,None stormwater_input,category,"Does the wastewater treatment plant specified in 'wwtp_name' treat water from a combined sewer system (i.e., a sewer system that collects both sewage and stormwater)?",[See Value Sets: vs_yne],[none],Not required,None influent_equilibrated,category,Is influent to the wastewater treatment plant specified in 'wwtp_name' ever stored prior to treatment to equilibrate or modulate the influent flow rate?,[See Value Sets: vs_yne],[none],Not required,None ,,,,,, Collection Method,,,,,, sample_type,category,"Type of sample collected, whether grab or composite. If composite, also provide the duration of sampling and type of composite, as listed in the Value Set (e.g., ""24-hr flow-weighted composite""). A grab sample is defined as an individual sample collected without compositing or adding other samples, regardless of whether the sample matrix is liquid wastewater or sludge.",[See Value Sets: vs_sample_type],[none],Required,None composite_freq,float,"Frequency of sub-sample collection (for composite samples only): for flow-weighted, the number of sub-samples collected per million gallons of flow; for time-weighted, the number of sub-samples per hour. Flow-weighted example: a value of 5 would indicate 5 sub-samples per million gallons, or 1 sub-sample per 200,000 gallons","[greater than or equal to 0]; [empty]",If flow-weighted composite: number per million gallons; if time-weighted or manual composite: number per hour,Not required,None sample_matrix,category,Wastewater matrix from which the sample was collected,[See Value Sets: vs_sample_matrix],[none],Required,None collection_storage_time,float,Duration of time from sample collection start time to time sample reached the lab. ,"[greater than or equal to 0]; [empty]",Hours,Not required,None collection_storage_temp,float,Temperature at which the sample was stored after collection and prior to reaching the lab,"[float]; [empty]",Celsius,Not required,None pretreatment,category,"Was the sample treated with any chemicals prior to reaching the lab? These could include chemicals, such as stabilizers, added to the sample or chemicals, such as chlorine, added to the wastewater treatment train upstream of the sample collection point. Do not be specify pasteurization here; it should be specified in the 'pasteurized' field.",[See Value Sets: vs_yn],[none],Required,"if 'pretreatment' is ""yes"", then specify in 'pretreatment_specify'" pretreatment_specify,string,"If 'pretreatment' is ""yes"", then specify the chemicals used",[string]; [empty],[none],Not required,"if 'pretreatment' is ""yes"", then 'pretreatment_specify' must have a non-empty field" ,,,,,, Processing Method,,,,,, solids_separation,category,"Process used to separate solid and liquid phases of the sample, either prior to or in the absence of the concentration method specified in 'concentration_method'",[See Value Sets: vs_solids_separation],[none],Not required,None concentration_method,category,Method used to concentrate the sample prior to analysis of the concentrate,[See Value Sets: vs_concentration_method],[none],Required,None extraction_method,category,Method used for nucleic acid extraction from the sample,[See Value Sets: vs_extraction_method],[none],Required,None pre_conc_storage_time,float,The approximate average duration of time between when samples reach the lab and when they are concentrated (if concentrated),"[greater than or equal to 0]; [empty]",Hours,Not required,None pre_conc_storage_temp,float,The storage temperature of samples after reaching the lab and prior to concentration (if concentrated),"[float]; [empty]",Celsius,Not required,None pre_ext_storage_time,float,The approximate average duration of time between when samples are concentrated (if concentrated) and when they are extracted,"[greater than or equal to 0]; [empty]",Hours,Not required,None pre_ext_storage_temp,float,The storage temperature of samples after concentration (if concentrated) and prior to extraction,"[float]; [empty]",Celsius,Not required,None tot_conc_vol,float,Total volume of sample concentrated (if concentrated); this total volume is not necessarily assayed and is not necessarily equal to the value specified in 'equiv_sewage_amt',"[greater than or equal to 0]; [empty]",mL,Not required,None ext_blank,category,Are extraction blanks included in the extraction process?,[See Value Sets: vs_yne],[none],Not required,None rec_eff_target_name,category,Name of the recovery efficiency control target that is spiked in,[See Value Sets: vs_rec_eff_target_name],[none],Required,"If 'rec_eff_percent' is equal to a value other than ""-1"", then this must have a non-empty value" rec_eff_spike_matrix,category,Matrix into which the recovery efficiency control target is spiked,[See Value Sets: vs_rec_eff_spike_matrix],[none],Required,"If 'rec_eff_target_name' has a non-empty value, then this must have a non-empty value" rec_eff_spike_conc,float,"Spike concentration, on average, of the recovery control on a per sample volume basis","[float]; [empty]",log10 copies/mL,Required,"If 'rec_eff_target_name' has a non-empty value, then this must have a non-empty value" pasteurized,category,Was the sample pasteurized?,[See Value Sets: vs_yn],[none],Required,None SARSCoV2 Quantification Method,,,,,, pcr_target,category,The target of the PCR quantification,[See Value Sets: vs_pcr_target],[none],Required,None pcr_gene_target,category,The PCR gene used to quantify PCR target,[See Value Sets: vs_pcr_gene_target],[none],Required,The value selected for pcr_gene_target should align with the value selected for pcr_target pcr_gene_target_ref,string,"A publication, website, or brief description of the PCR gene target used",[string],[none],Required,None pcr_type,category,The type of PCR used to quantify the PCR target,[See Value Sets: vs_pcr_type],[none],Required,"If 'pcr_target' is not ""sars-cov-2"", ""delta"", ""omicron"", ""hMPXV"", ""hMPXV Clade I"", or ""hMPXV Clade II"" then this must be a type of digital PCR, i.e. ""ddpcr"", ""qiagen dpcr"", ""fluidigm dpcr"", ""life technologies dpcr"", ""raindance dpcr"", or ""dpcr""" lod_ref,string,"A publication, website, or brief description of the method used to calculate the limit of detection",[string],[none],Required,None hum_frac_target_mic,category,Name of microbial target used to estimate human fecal content,[See Value Sets: vs_hum_frac_target_mic],[none],Not required,"If 'hum_frac_mic_conc' has a non-empty value, then this must have a non-empty value" hum_frac_target_mic_ref,string,"A publication, website, or brief description of the microbial target specified in 'hum_frac_target_mic'","[string]; [empty]",[none],Not required,"If 'hum_frac_mic_conc' has a non-empty value, then this must have a non-empty value" hum_frac_target_chem,category,Name of chemical compound used to estimate human fecal content,[See Value Sets: vs_hum_frac_target_chem],[none],Not required,"If 'hum_frac_chem_conc' has a non-empty value, then this must have a non-empty value" hum_frac_target_chem_ref,string,"A publication, website, or brief description of the chemical compound specified in 'hum_frac_target_chem'","[string]; [empty]",[none],Not required,"If 'hum_frac_chem_conc' has a non-empty value, then this must have a non-empty value" other_norm_name,category,Name of a target or compound not specified in 'hum_frac_target_mic' or 'hum_frac_target_chem' used to estimate human fecal content,[See Value Sets: vs_other_norm_name],[none],Not required,"If 'other_norm_conc' has a non-empty value, then this must have a non-empty value" other_norm_ref,string,"A publication, website, or brief description of the target or compound specified in 'other_norm_name'","[string]; [empty]",[none],Not required,"If 'other_norm_conc' has a non-empty value, then this must have a non-empty value" quant_stan_type,category,The type of nucleic acid used as a standard for the PCR target quantification,[See Value Sets: vs_quant_stan_type],[none],Required,None stan_ref,string,"A publication, website, or brief description of the quantitative standard material used",[string],[none],Required,None inhibition_method,string,"A publication, website, or brief description of the method used to evaluate molecular inhibition","[string]; none (if inhibition not tested)",[none],Required,None num_no_target_control,category,Number of no-template controls (NTC) per instrument run,[See Value Sets: vs_num_no_target_control],[none],Required,None ,,,,,, Sample,,,,,, sample_collect_date,date ([yyyy]-[mm]-[dd]),"The date of sample collection; for composite samples, specify the date on which sample collection began",[date not after tomorrow's date],[none],Required,None sample_collect_time,"time, 24-hr ([hh]:[mm])","The local time of sample collection; for composite samples, specify the time at which sample collection began",[time],[none],Required,None time_zone,time zone (UTC-[hh]:[mm]),"Current local time zone corresponding to the time specified in 'sample_collect_time', represented as a UTC time offset (e.g., UTC-06:00)",[time zone],[none],Not required,None flow_rate,float,"Wastewater volumetric flow rate at the sample collection location over the 24-hr period during which the sample was collected. If only an instantaneous flow measurement is available, it may be reported in units of million gallons per day. ","[greater than or equal to 0]; [empty]",Million gallons per day (MGD),Required. ,"If 'sars_cov2_units' is on a per volume wastewater basis (e.g., copies/l wastewater), then this must have a non-empty value (i.e., measurements of wastewater solids are permitted empty values for 'flow_rate'). If 'hum_frac_mic_conc' has a non-empty value and it is not possible to measure flow rate at all, then this may have an empty value." ph,float,"pH of wastewater sample (if sludge, pH of influent at time of collection)","[float]; [empty]",pH units,Not required,None conductivity,float,"Specific conductivity of wastewater sample (if sludge, conductivity of influent at time of collection)","[greater than or equal to 0]; [empty]",microsiemens/cm,Not required,None tss,float,"Total suspended solids of raw (or, if unavailable, post-grit removal) wastewater ","[greater than or equal to 0]; [empty]",mg/L,Not required,None collection_water_temp,float,Sample temperature at time of collection,"[greater than or equal to 0]; [empty]",Celsius,Not required,None equiv_sewage_amt,float,Equivalent unconcentrated volume of wastewater or mass of sludge in PCR reaction,"[greater than or equal to 0]; [empty]",mL wastewater or g sludge,Not required,None sample_id,"jurisdiction id (a string 20 characters or less, containing only numbers, English alphabetic characters, underscores, and hyphens; white space is not allowed; not case sensitive)",An ID assigned to a wastewater sample. It must be unique for this NWSS reporting jurisdiction. Wastewater samples that are split and measured by different labs should have the same sample ID but different lab IDs. Wastewater samples for which multiple PCR targets are measured should also have the same sample ID. Note: do not include PII in this field.,[sample id],[none],Required,None lab_id,"jurisdiction id (a string 20 characters or less, containing only numbers, English alphabetic characters, underscores, and hyphens; white space is not allowed; not case sensitive)","An ID assigned to a testing lab. It must be unique across labs used for this NWSS reporting jurisdiction's testing. If the same lab is used across multiple NWSS reporting jurisdictions, each NWSS reporting jurisdiction may assign that lab a different lab ID. Note: including PII in this field is discouraged.",[lab id],[none],Required,None qc_ignore,category,"Should the DCIPHER QC report ignore this sample? The purpose of this field is to allow for removal of samples from the QC report so that samples with unresolvable QC issues do not clutter the report. This field only affects whether samples are shown in the QC report, not how they are otherwise processed or analyzed. If the value is ""yes"", then this sample will be excluded from the QC report. If the value is ""no"" or [empty], then this sample will be included in the QC report for up to 90 days. Samples will only be included in the QC report for up to 90 days, regardless of the value specified in ‘qc_ignoreâ€?",[See Value Sets: vs_yne],[none],Not required,None dashboard_ignore,category,"Should the DCIPHER dashboard ignore this sample? If the value is ""yes"", then this sample will be excluded from the dashboard. If the value is ""no"" or [empty], then this sample will be included in the dashboard. In addition to excluding the sample from the DCIPHER dashboard, this field will also prevent the sample from public data release.",[See Value Sets: vs_yne],[none],Not required,None analysis_ignore,category,"Should DCIPHER data analysis ignore this sample? If the value is ""yes"", then this sample will be excluded from DCIPHER data analysis, which will also cause it to be excluded from the dashboard. If the value is ""no"" or [empty], then this sample will be included in DCIPHER data analysis.",[See Value Sets: vs_yne],[none],Not required,None ,,,,,, SARSCoV2 Quantification Results,,,,,, test_result_date,date ([yyyy]-[mm]-[dd]),The date on which this PCR target measurement was made,[date greater than sample collect date and less than DCIPHER upload date],[none],Required,None pcr_target_units,category,Units of PCR target sample concentration,[See Value Sets: vs_mic_chem_units],[none],Required,The units should relate to the PCR target indicated in pcr_target pcr_target_avg_conc,float,"Concentration of the PCR target back-calculated to unconcentrated sample basis; enter ""0"" if no amplification occurred, using the definition of amplification described in 'ntc_amplify'; otherwise, enter the estimated concentration; do not adjust for matrix recovery efficiency. Only the technical or biological replicate concentrations for the same pcr_gene_target should be averaged. For example, if there are three replicates of a given PCR target, average those concentrations and report the average value only. Do not include the three individual concentrations. Different pcr_gene_target concentrations should not be averaged for this field (e.g., do not average concentrations for N1 and N2). ","[any float other than 0]; 0 (if no amplification observed)",[units specified in 'pcr_target_units'],Required,The concentration should relate to the PCR target indicated in pcr_target pcr_target_std_error,float,"Standard error (SE) of the PCR target in wastewater sample, or best estimate that is consistently available. If sample replicates are always performed, use SE of sample replicates; else, if processing replicates are always performed, use SE of processing replicates; else, if qPCR is performed, use SE of PCR replicates; else, if digital PCR is performed, use error from multiple replicates if available, and Poisson error if not","[greater than or equal to 0]; -1 (if cannot be calculated, such as when no amplification observed)",[units specified in 'pcr_target_units'],Not required,The standard error should relate to the PCR target indicated in pcr_target pcr_target_cl_95_lo,float,"Lower bound of 95% confidence interval of the PCR target in wastewater sample, or best estimate that is consistently available. Follow the same hierarchy as described for standard error. (Note: 'cl' stands for confidence limit)","[any float other than -1]; -1 (if cannot be calculated, such as when no amplification observed)",[units specified in 'pcr_target_units'],Not required,The lower bound of the 95% confidence interval should relate to the PCR target indicated in pcr_target pcr_target_cl_95_up,float,"Upper bound of 95% confidence interval of the PCR target in wastewater sample, or best estimate that is consistently available. Follow the same hierarchy as described for standard error. (Note: 'cl' stands for confidence limit)","[any float other than -1]; -1 (if cannot be calculated, such as when no amplification observed)",[units specified in 'pcr_target_units'],Not required,The upper bound of the 95% confidence interval should relate to the PCR target indicated in pcr_target pcr_target_below_lod,category,Was the concentration of the PCR target below the limit of detection?,[See Value Sets: vs_yne],[none],Not required,Whether the concentration was below the LOD should be based on the LOD of the PCR target indicated in lod_sewage lod_sewage,float,PCR target limit of detection back-calculated to unconcentrated sample basis,[float],[units specified in 'pcr_target_units'],Required,The LOD should relate to the PCR target indicated in pcr_target ntc_amplify,category,"For qPCR, did any no-template controls on this instrument run have a Ct value less than 40? For ddPCR, did any no-template controls on this instrument run have 3 or more positive droplets?",[See Value Sets: vs_yn],[none],Required,None rec_eff_percent,float,"Percent of spiked recovery control, specified in 'rec_eff_target_name', that was recovered","[greater than or equal to 0.0]; -1 (if not tested)",percent,Required,None inhibition_detect,category,Was molecular inhibition detected?,[See Value Sets: vs_ynn],[none],Required,None inhibition_adjust,category,Was inhibition incorporated into the PCR target concentration calculation?,[See Value Sets: vs_yne],[none],Required,"If 'inhibition_detect' is ""yes"", then this must have a non-empty value" hum_frac_mic_conc,float,Concentration of microbial target specified in 'hum_frac_target_mic'; follow the same guidelines outlined for 'pcr_target_avg_conc',"[float]; [empty]",[units specified in 'hum_frac_mic_unit'],Not required,"If 'hum_frac_mic_unit' has a non-empty value, then this must have a non-empty value" hum_frac_mic_unit,category,Concentration units of microbial target specified in 'hum_frac_target_mic',[See Value Sets: vs_mic_chem_units_e],[none],Not required,"If 'hum_frac_mic_conc' has a non-empty value, then this must have a non-empty value" hum_frac_chem_conc,float,Concentration of chemical target specified in 'hum_frac_target_chem',"[float]; [empty]",[units specified in 'hum_frac_chem_unit'],Not required,"If 'hum_frac_chem_unit' has a non-empty value, then this must have a non-empty value" hum_frac_chem_unit,category,Concentration units of chemical target specified in 'hum_frac_target_chem',[See Value Sets: vs_mic_chem_units_e],[none],Not required,"If 'hum_frac_chem_conc' has a non-empty value, then this must have a non-empty value" other_norm_conc,float,Concentration of target spcified in 'other_norm_name',"[float]; [empty]",[units specified in 'other_norm_conc'],Not required,"If 'other_norm_unit' has a non-empty value, then this must have a non-empty value" other_norm_unit,category,Concentration units of target spcified in 'other_norm_name',[See Value Sets: vs_mic_chem_units_e],[none],Not required,"If 'other_norm_conc' has a non-empty value, then this must have a non-empty value" quality_flag,category,Does this observation have quality control issues? ,[See Value Sets: vs_yne],[none],Not required,None major_lab_method,integer,"A number used to distinguish major lab methods at the reporting jurisdiction level. Differences in lab methods may limit the comparability of PCR target concentrations. Reporting jurisdictions should use this field to indicate that lab methods are sufficiently different that caution should be used when comparing PCR target concentrations across them. These differences may result from any lab processing or quantification steps, including different PCR gene targets. Note that 'major_lab_method' may be the same for different laboratories if the reporting jurisdiction believes concentrations measured by those laboratories are comparable; conversely, 'major_lab_method' may be different for the same laboratory if changes made to the laboratory protocol rendered concentrations not comparable.","[greater than or equal to 0]; [empty]",[none],Required,None major_lab_method_desc,string,"Brief description of the basis for assigning a ‘major_lab_methodâ€? e.g., ""this lab uses a lab method distinct from other labs in this reporting jurisdiction"", ""this lab changed lab methods - new concentration method"" or ""lab method is same as other labs in this reporting jurisdiction but performance differs""","[string]; [empty]",[none],Required,None ¹ú²ú¾«Æ·¾Ã¾Ã¾Ã¾Ã¾ÃÒ»¼¶Ã«Æ¬