Notice to Readers: Update: West Nile Virus Isolated from Mosquitoes --- New York, 2000
During January--February 2000, three pools of overwintering
Culex spp. mosquitoes collected at Fort Totten, New York, were positive for West Nile
Virus (WNV) RNA by real-time reverse transcriptase polymerase chain reaction
(RT-PCR) assay (TaqMan™, Perkin-Elmer Biosystems, Foster City, California*)
(1). Additional testing to detect the presence of intact virus was performed but was negative as
of March 10, 2000. Since then, one of the pools that was positive by RT-PCR yielded a
live WNV isolate in a fluid vero cell culture system and confirmed by West
Nile-specific monoclonal antibody staining of virus infected cells and virus gene
sequencing. Studies of this isolate continue.
Reference
CDC. Update: surveillance for West Nile Virus in overwintering mosquitoes---New
York, 2000. MMWR 2000;49:178--9.
* Use of trade names is for identification only and does not imply endorsement by CDC
or the U.S. Department of Health and Human Services.
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